Overview
We performed deep mutational scans to measure the impact on ACE2 binding of all single amino-acid mutations in the Omicron BQ.1.1 and XBB.1.5 variant RBDs, and compare mutational effects to those in previously queried SARS-CoV-2 variants Wuhan-Hu-1, Eta, Alpha, Beta, Delta, Omicron BA.1, and BA.2 from previously published studies here and here.
Here, we link to two interactive visualizations that enable exploration of the data from these deep mutational scans across SARS_CoV-2 variants.
Instructions
We have made two tools to help visualize the data from our deep mutational scans:
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A set of interactive heatmaps that you can use to explore the change in ACE2-binding affinity (\(\Delta\)log10 \(K_D\)) or the change in RBD expression (log10(MFI)) caused by mutations in each RBD variant. To use this tool, click here.
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An interactive widget that you can use to visualize epistatic shifts in mutational effects on ACE2-binding affinity (-log10 \(K_D\)) between variant RBDs. To use this tool, click here.
Data
If you are interested in the raw data from our study, you can find the ACE2-binding affinity (-log10 \(K\_D\)) and RBD expression (log10(MFI)) measurements from each experiment here. You can find the data used to plot the epistatic shifts between variant backgrounds here.